Issue 67, 2016
From the journal:

Chemical Communications

3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy†

Bin-Bin Pan,a   Feng Yang,a   Yansheng Ye,b   Qiong Wu,b   Conggang Li,*b   Thomas Huber*c  and  Xun-Cheng Su*a  

* Corresponding authors

a State Key Laboratory and Research Institute of Elemento-Organic Chemistry, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China
E-mail: xunchegnsu@nankai.edu.cn

b Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China
E-mail: conggangli@wipm.ac.cn

c Research School of Chemistry, Australian National University, Canberra, Australia
E-mail: t.huber@anu.edu.au

Abstract

Determining the three-dimensional structure of a protein in living cells remains particularly challenging. We demonstrated that the integration of site-specific tagging proteins and GPS-Rosetta calculations provides a fast and effective way of determining the structures of proteins in living cells, and in principle the interactions and dynamics of protein–ligand complexes.

Graphical abstract: 3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

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Article information

DOI
https://doi.org/10.1039/C6CC05490K
Article type
Communication
Submitted
04 Jul 2016
Accepted
20 Jul 2016
First published
20 Jul 2016

Chem. Commun., 2016,52, 10237-10240

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3D structure determination of a protein in living cells using paramagnetic NMR spectroscopy

B. Pan, F. Yang, Y. Ye, Q. Wu, C. Li, T. Huber and X. Su, Chem. Commun., 2016, 52, 10237 DOI: 10.1039/C6CC05490K

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