Issue 90, 2016

Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor

Abstract

Molecular rotors have emerged as versatile probes of microscopic viscosity in lipid bilayers, although it has proved difficult to find probes that stain both phases equally in phase-separated bilayers. Here, we investigate the use of a membrane-targeting viscosity-sensitive fluorophore based on a thiophene moiety with equal affinity for ordered and disordered lipid domains to probe ordering and viscosity within artificial lipid bilayers and live cell plasma membranes.

Graphical abstract: Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor

Supplementary files

Article information

Article type
Communication
Submitted
19 Jul 2016
Accepted
06 Oct 2016
First published
07 Oct 2016

Chem. Commun., 2016,52, 13269-13272

Imaging plasma membrane phase behaviour in live cells using a thiophene-based molecular rotor

M. R. Dent, I. López-Duarte, C. J. Dickson, P. Chairatana, H. L. Anderson, I. R. Gould, D. Wylie, A. Vyšniauskas, N. J. Brooks and M. K. Kuimova, Chem. Commun., 2016, 52, 13269 DOI: 10.1039/C6CC05954F

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