Steric hindrance inhibition of strand displacement for homogeneous and signal-on fluorescence detection of human serum antibodies

Abstract

Based on a new steric hindrance inhibition of the DNA strand displacement strategy, we report the design of a robust fluorescence signal-on method for homogeneous and sensitive detection of antibodies from human serum samples. Such a steric hindrance effect leads to sensitive detection of the target antibodies with a detection limit of 5.6 nM. In addition, the developed sensing approach shows high selectivity against other interference proteins and the detection of the target antibodies in human sera by this method is also verified.