Issue 4, 2017

Efficacious fluorescence turn-on probe for high-contrast imaging of human cells overexpressing quinone reductase activity

Abstract

We report a new turn-on substrate probe whose intense fluorescent reporter signature is selectively provided upon probe activation by the cancer-associated oxidoreductase, hNQO1. The extremely high fluorescence turn-on of the probe was utilized to generate fluorescence microscope images of hNQO1-expressing, tumor-derived colorectal and ovarian cancer cells with unprecedented positive signal-to-negative background ratios (PNRs), a key step toward probe application in guided surgical removal of diseased tissues.

Graphical abstract: Efficacious fluorescence turn-on probe for high-contrast imaging of human cells overexpressing quinone reductase activity

Supplementary files

Article information

Article type
Communication
Submitted
14 Oct 2016
Accepted
12 Dec 2016
First published
12 Dec 2016

Chem. Commun., 2017,53, 783-786

Efficacious fluorescence turn-on probe for high-contrast imaging of human cells overexpressing quinone reductase activity

Q. A. Best, B. Prasai, A. Rouillere, A. E. Johnson and R. L. McCarley, Chem. Commun., 2017, 53, 783 DOI: 10.1039/C6CC08306D

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