Photoactivation of the Ni-SIr state to the Ni-SIa state in [NiFe] hydrogenase: FT-IR study on the light reactivity of the ready Ni-SIr state and as-isolated enzyme revisited

Hulin Tai; Liyang Xu; Seiya Inoue; Koji Nishikawa; Yoshiki Higuchi; Shun Hirota

doi:10.1039/C6CP04628B

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Photoactivation of the Ni-SI_r state to the Ni-SI_a state in [NiFe] hydrogenase: FT-IR study on the light reactivity of the ready Ni-SI_r state and as-isolated enzyme revisited†

Hulin Tai,^ab Liyang Xu,^a Seiya Inoue,^c Koji Nishikawa,^c Yoshiki Higuchi^bc and Shun Hirota*^ab

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* Corresponding authors

^a Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma-shi, Nara 630-0192, Japan
E-mail: hirota@ms.naist.jp
Fax: +81-743-72-6119
Tel: +81-743-72-6110

^b CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

^c Graduate School of Life Science, University of Hyogo, 3-2-1 Koto Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan

Abstract

The Ni-SI_r state of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F was photoactivated to its Ni-SI_a state by Ar⁺ laser irradiation at 514.5 nm, whereas the Ni-SL state was light induced from a newly identified state, which was less active than any other identified state and existed in the “as-isolated” enzyme.

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Article information

DOI: https://doi.org/10.1039/C6CP04628B
Article type: Communication
Submitted: 03 Jul 2016
Accepted: 15 Jul 2016
First published: 15 Jul 2016

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Phys. Chem. Chem. Phys., 2016,18, 22025-22030

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Photoactivation of the Ni-SI_r state to the Ni-SI_a state in [NiFe] hydrogenase: FT-IR study on the light reactivity of the ready Ni-SI_r state and as-isolated enzyme revisited

H. Tai, L. Xu, S. Inoue, K. Nishikawa, Y. Higuchi and S. Hirota, Phys. Chem. Chem. Phys., 2016, 18, 22025 DOI: 10.1039/C6CP04628B

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