Issue 11, 2016

Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

Abstract

Despite recent advances in microfluidic-based integrated diagnostic systems, the sample introduction interface, especially with regards to large volume samples, has often been neglected. We present a sample introduction interface that allows direct on-chip processing of crude stool samples for the detection of Helicobacter pylori (H. pylori). The principle of IFAST (immiscible filtration assisted by surface tension) was adapted to include a large volume sample chamber with a septum-based interface for stool sample introduction. Solid chaotropic salt and dry superparamagnetic particles (PMPs) could be stored on-chip and reconstituted upon sample addition, simplifying the process of release of DNA from H. pylori cells and its binding to the PMPs. Finally, the PMPs were pulled via a magnet through a washing chamber containing an immiscible oil solution and into an elution chamber where the DNA was released into aqueous media for subsequent analysis. The entire process required only 7 min while enabling a 40-fold reduction in working volume from crude biological samples. The combination of a real-world interface and rapid DNA extraction offers the potential for the methodology to be used in point-of-care (POC) devices.

Graphical abstract: Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

Supplementary files

Article information

Article type
Paper
Submitted
18 Feb 2016
Accepted
28 Apr 2016
First published
10 May 2016
This article is Open Access
Creative Commons BY license

Lab Chip, 2016,16, 2108-2115

Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

O. Mosley, L. Melling, M. D. Tarn, C. Kemp, M. M. N. Esfahani, N. Pamme and K. J. Shaw, Lab Chip, 2016, 16, 2108 DOI: 10.1039/C6LC00228E

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