Issue 39, 2016

An ultrasensitive SERS sensor for simultaneous detection of multiple cancer-related miRNAs

Abstract

Simultaneous detection of multiple trace cancer associated serum miRNA biomarkers is considered as a feasible method for early cancer screening and diagnosis. In the present work, an ultrasensitive SERS sensor was prepared based on an Ag nanorod array SERS substrate by assembling special hairpin-shaped molecular beacons (MBs) for the detection of multiple lung cancer-related miRNA biomarkers. The portable SERS sensor exhibits excellent performance for the qualitative and quantitative detection of miRNAs, with advantages of ultra-sensitivity, good specificity, uniformity, reproducibility and stability, as well as remarkable reusability. By monitoring the SERS signal quenching of the MBs in the presence of target miRNA biomarkers, three lung cancer related-miRNAs (miRNA-21, miRNA-486, and miRNA-375) in buffer and human serum were simultaneously assayed using the SERS sensor array, and the limits of detection of the three miRNAs in human serum are 393 aM, 176 aM, and 144 aM, respectively. The reliable results demonstrate that the proposed SERS sensor array can be a promising candidate with great potential for the screening and clinical diagnosis of cancer in the early stage.

Graphical abstract: An ultrasensitive SERS sensor for simultaneous detection of multiple cancer-related miRNAs

Supplementary files

Article information

Article type
Paper
Submitted
12 Jul 2016
Accepted
05 Sep 2016
First published
05 Sep 2016

Nanoscale, 2016,8, 17365-17373

An ultrasensitive SERS sensor for simultaneous detection of multiple cancer-related miRNAs

C. Y. Song, Y. J. Yang, B. Y. Yang, Y. Z. Sun, Y. P. Zhao and L. H. Wang, Nanoscale, 2016, 8, 17365 DOI: 10.1039/C6NR05504D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements