Issue 32, 2016

Site-selective incorporation and ligation of protein aldehydes

Abstract

The incorporation of aldehyde handles into proteins, and subsequent chemical reactions thereof, is rapidly proving to be an effective way of generating homogeneous, covalently linked protein constructs that can display a vast array of functionality. In this review, we discuss methods for introducing aldehydes into target proteins, and summarise the ligation strategies for site-selective modification of proteins containing this class of functional handles.

Graphical abstract: Site-selective incorporation and ligation of protein aldehydes

Article information

Article type
Review Article
Submitted
11 Apr 2016
Accepted
30 Jun 2016
First published
30 Jun 2016
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2016,14, 7622-7638

Author version available

Site-selective incorporation and ligation of protein aldehydes

R. J. Spears and M. A. Fascione, Org. Biomol. Chem., 2016, 14, 7622 DOI: 10.1039/C6OB00778C

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