Issue 61, 2016

Antibody conjugated metal nanoparticle decorated graphene sheets for a mycotoxin sensor

Abstract

We demonstrate the fabrication of a label-free immunosensor for electrochemical detection of mycotoxin i.e. aflatoxin B1 (AFB1) using nickel nanoparticle decorated reduced graphene oxide (rGO–Ni NPs) sheets. The rGO–Ni NPs have been electrophoretically deposited onto an indium tin oxide (ITO) coated glass electrode. This fabricated immunosensor is sensitive to 0.16 ng mL−1 concentration of AFB1 in phosphate buffer solution. It exhibits a lower limit of detection when compared to the HPLC standard method (2.5 ng mL−1) and shows minimal interference with another food toxin (ochratoxin A). Electron microscopy, Fourier transform infrared, UV-visible spectroscopy and electrochemical techniques were utilized to confirm selective decoration of Ni NPs on the rGO sheets and to investigate the chemical and electrochemical properties of the nanocomposite. The highly crystalline properties of the rGO–Ni NPs sheets along with the excellent electro-catalytic properties of the Ni NPs result in a higher heterogeneous electron transfer rate indicating the enhanced sensitivity (129.6 μA ng−1 mL cm−2) of the device. The favorable environment for antibody conjugation on the surface of the rGO–Ni NPs sheets offers a low limit-of-detection (0.16 ng mL−1) for AFB1.

Graphical abstract: Antibody conjugated metal nanoparticle decorated graphene sheets for a mycotoxin sensor

Article information

Article type
Paper
Submitted
19 Feb 2016
Accepted
06 Jun 2016
First published
08 Jun 2016

RSC Adv., 2016,6, 56518-56526

Antibody conjugated metal nanoparticle decorated graphene sheets for a mycotoxin sensor

S. Srivastava, V. Kumar, K. Arora, C. Singh, Md. A. Ali, N. K. Puri and B. D. Malhotra, RSC Adv., 2016, 6, 56518 DOI: 10.1039/C6RA04469G

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