Issue 56, 2016, Issue in Progress

Rhodamine-based ratiometric fluorescent probes based on excitation energy transfer mechanisms: construction and applications in ratiometric sensing

Abstract

Ratiometric fluorescent probes allow the simultaneous measurement of two fluorescence signals at different wavelengths followed by calculation of their intensity ratio, which can provide more precise measurement results than intensity-based fluorescent probes. Excitation energy transfer is widely used in the design of ratiometric fluorescent probes. Rhodamine is a convenient platform for the construction of “OFF–ON” ratiometric chemosensors. Rhodamine-based ratiometric fluorescent probes based on the excitation energy transfer mechanism can be constructed by conjugated or non-conjugated connections with other chromophores. In this review, we summarized the recent advances regarding rhodamine-based ratiometric fluorescent probes based on excitation energy transfer. We reviewed these probes according to the classification of “through-space” and “through-bond” probes; we focused on the contributions of different donor fluorophores and the types of connections between the energy donors and acceptors.

Graphical abstract: Rhodamine-based ratiometric fluorescent probes based on excitation energy transfer mechanisms: construction and applications in ratiometric sensing

Article information

Article type
Review Article
Submitted
16 Mar 2016
Accepted
09 May 2016
First published
11 May 2016

RSC Adv., 2016,6, 50732-50760

Rhodamine-based ratiometric fluorescent probes based on excitation energy transfer mechanisms: construction and applications in ratiometric sensing

R. Zhang, F. Yan, Y. Huang, D. Kong, Q. Ye, J. Xu and L. Chen, RSC Adv., 2016, 6, 50732 DOI: 10.1039/C6RA06956H

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