Issue 93, 2016, Issue in Progress

Electrochemical sensor for individual and simultaneous determination of guanine and adenine in biological fluids and in DNA based on a nano-In–ceria modified glassy carbon paste electrode

Abstract

A novel, simple and sensitive electrochemical method for individual and simultaneous determination and direct electro-oxidation behaviors of guanine (G) and adenine (A) was developed using an In doped ceria nanoparticle modified glassy carbon paste electrode (In–CeO2/GCPE). Scanning electron microscopy (SEM) and electrochemical techniques were employed for characterization of the fabricated modified electrode. The electrochemical sensor exhibits a potent and persistent electro-oxidation behavior followed by well-separated oxidation peaks toward G and A. Electrochemical performances related to the electro-oxidation of G and A at the nano-In–CeO2NPs/GCPE were investigated, showing that their peak currents were greatly enhanced due to the catalytic effect of In doped ceria. The prepared In–CeO2NPs/GCPE showed high selectivity and sensitivity for G and A oxidation over uric acid (UA). A linear range of 0.07–34 μM with a detection limit of 1.19 × 10−8 M for G and 1.96–88.2 μM with a detection limit of 2.86 × 10−8 M for A were achieved. The proposed sensor was used for individual and simultaneous determination of G and A in biological fluids and in ssDNA samples. The value of (G + C)/(A + T) in DNA was calculated to be 0.75.

Graphical abstract: Electrochemical sensor for individual and simultaneous determination of guanine and adenine in biological fluids and in DNA based on a nano-In–ceria modified glassy carbon paste electrode

Supplementary files

Article information

Article type
Paper
Submitted
26 May 2016
Accepted
09 Sep 2016
First published
20 Sep 2016

RSC Adv., 2016,6, 90220-90231

Electrochemical sensor for individual and simultaneous determination of guanine and adenine in biological fluids and in DNA based on a nano-In–ceria modified glassy carbon paste electrode

H. Ibrahim, Y. Temerk and N. Farhan, RSC Adv., 2016, 6, 90220 DOI: 10.1039/C6RA13704K

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