Issue 1, 2017

Dual-emissive nanoarchitecture of lanthanide-complex-modified silica particles for in vivo ratiometric time-gated luminescence imaging of hypochlorous acid

Abstract

We have developed a ratiometric time-gated luminescence sensory system for in vivo imaging of hypochlorous acid (HClO) by preparing a dual-emissive nanoarchitecture of europium- and terbium-complex-modified silica nanoparticles. The design of this nanoarchitecture is based on our new finding that the strong, long-lived luminescence of the β-diketonate–Eu3+ complex can be rapidly and selectively quenched by HClO. Therefore, the β-diketonate–Eu3+ complex was decorated on the surface of the silica nanoparticles for responding to HClO, while a HClO-insensitive luminescent terbium complex was immobilized in the inner solid core of the nanoparticles to serve as an internal standard. This nanosensing probe combines the advantages of both ratiometric and time-gated detection modes to afford high accuracy and sensitivity. Upon exposure to HClO, the nanoprobe displayed a remarkable luminescence color change from red to green, and the intensity ratio of the green over the red luminescence (I539/I607) showed a rapid, sensitive and selective response to HClO. Additionally, the feasibility of using the nanoprobe for intracellular detection of exogenous and endogenous HClO and for real-time mapping of HClO in small laboratory animals has been demonstrated via ratiometric time-gated luminescence imaging microscopy. The results reveal that the constructed nanoarchitecture cloud is a favorable and useful sensing probe for the real-time imaging of HClO in vivo with high specificity and contrast.

Graphical abstract: Dual-emissive nanoarchitecture of lanthanide-complex-modified silica particles for in vivo ratiometric time-gated luminescence imaging of hypochlorous acid

Supplementary files

Article information

Article type
Edge Article
Submitted
20 May 2016
Accepted
25 Jul 2016
First published
29 Jul 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2017,8, 150-159

Dual-emissive nanoarchitecture of lanthanide-complex-modified silica particles for in vivo ratiometric time-gated luminescence imaging of hypochlorous acid

H. Ma, B. Song, Y. Wang, D. Cong, Y. Jiang and J. Yuan, Chem. Sci., 2017, 8, 150 DOI: 10.1039/C6SC02243J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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