Issue 27, 2017
From the journal:

Chemical Communications

Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase†

Li-Juan Wang,‡a   Zi-Yue Wang,‡a   Qianyi Zhang,b   Bo Tang ORCID logo *a  and  Chun-Yang Zhang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn, tangb@sdnu.edu.cn
Fax: +86 0531-82615258
Tel: +86 0531-86186033

b Nantou High School Shenzhen, Shenzhen, China

Abstract

We develop a new fluorescence method for real-time monitoring of thymine DNA glycosylase (TDG) activity through cyclic enzymatic repairing-mediated dual-signal amplification. This method exhibits excellent sensitivity with a detection limit of 5.6 × 10−7 U μL−1, and it can be used to determine kinetic parameters and quantify TDG activity from even single cancer cells.

Graphical abstract: Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C7CC00946A
Article type
Communication
Submitted
06 Feb 2017
Accepted
13 Mar 2017
First published
13 Mar 2017

Chem. Commun., 2017,53, 3878-3881

Permissions

Request permissions

Cyclic enzymatic repairing-mediated dual-signal amplification for real-time monitoring of thymine DNA glycosylase

L. Wang, Z. Wang, Q. Zhang, B. Tang and C. Zhang, Chem. Commun., 2017, 53, 3878 DOI: 10.1039/C7CC00946A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements