Issue 50, 2017
From the journal:

Chemical Communications

Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy†

E. Kozma,a   G. Estrada Girona, ORCID logo b   G. Paci, ORCID logo b   E. A. Lemkeb  and  P. Kele ORCID logo *a  

* Corresponding authors

a “Lendület” Chemical Biology Research Group, Institute of Organic Chemistry, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok krt. 2, 1117 Budapest, Hungary
E-mail: kele.peter@ttk.mta.hu

b Structural and Computational Biology Unit, Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany

Abstract

A series of double-fluorogenic siliconrhodamine probes were synthesized. These tetrazine-functionalized, membrane-permeable labels allowed site-specific bioorthogonal tagging of genetically manipulated intracellular proteins and subsequent imaging using super-resolution microscopy.

Graphical abstract: Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy

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Article information

DOI
https://doi.org/10.1039/C7CC02212C
Article type
Communication
Submitted
23 Mar 2017
Accepted
11 May 2017
First published
11 May 2017
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2017,53, 6696-6699

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Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy

E. Kozma, G. Estrada Girona, G. Paci, E. A. Lemke and P. Kele, Chem. Commun., 2017, 53, 6696 DOI: 10.1039/C7CC02212C

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