Issue 15, 2018
From the journal:

Chemical Communications

Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe†

Firoj Ali,‡a   Sunil Aute,‡a   Sreejesh Sreedharan,‡b   H. A. Anila,a   Hiwa K. Saeed,b   Carl G. Smythe,*c   Jim A. Thomas ORCID logo *b  and  Amitava Das ORCID logo *acd  

* Corresponding authors

a Organic Chemistry Division, CSIR-National Chemical Laboratory, Pune – 411008, India

b Department of Chemistry, University of Sheffield, Sheffield, UK
E-mail: james.thomas@sheffield.ac.uk

c Department of Biomedical Science, University of Sheffield, Sheffield, UK
Web: http://mailto:c.g.w.smythe@sheffield.ac.uk

d CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar, Gujarat, India
E-mail: a.das@csmcri.res.in

Abstract

BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.

Graphical abstract: Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe

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Article information

DOI
https://doi.org/10.1039/C7CC09433G
Article type
Communication
Submitted
12 Dec 2017
Accepted
25 Jan 2018
First published
31 Jan 2018

Chem. Commun., 2018,54, 1849-1852

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Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe

F. Ali, S. Aute, S. Sreedharan, H. A. Anila, H. K. Saeed, C. G. Smythe, J. A. Thomas and A. Das, Chem. Commun., 2018, 54, 1849 DOI: 10.1039/C7CC09433G

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