Issue 13, 2018

Cell-size confinement effect on protein diffusion in crowded poly(ethylene)glycol solution

Abstract

Micrometric membrane confinements and macromolecular crowding of cytoplasm are key factors that regulate molecular diffusion in live cells. Previous studies have shown that macromolecular crowding delays molecular diffusion. However, the effect of cell-size confinement on diffusion in the crowding environment is yet to be elucidated. Using fluorescence correlation spectroscopy (FCS), we analyzed protein diffusion in microdroplets containing polymer solution covered with lipid membranes that mimic cells. As a result, we found that a synergistic condition of crowding and micrometric confinement results in accelerated protein diffusion on a sub-millisecond time scale. This acceleration rate strongly depended on the size of the confined space and the degree of crowding. These findings indicate that cell-size confinement supports protein diffusion in highly crowded cytoplasm.

Graphical abstract: Cell-size confinement effect on protein diffusion in crowded poly(ethylene)glycol solution

Supplementary files

Article information

Article type
Paper
Submitted
07 Dec 2017
Accepted
09 Mar 2018
First published
09 Mar 2018

Phys. Chem. Chem. Phys., 2018,20, 8842-8847

Cell-size confinement effect on protein diffusion in crowded poly(ethylene)glycol solution

C. Watanabe and M. Yanagisawa, Phys. Chem. Chem. Phys., 2018, 20, 8842 DOI: 10.1039/C7CP08199E

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