Issue 10, 2017

A new fluorescent sensor containing glutamic acid for Fe3+ and its resulting complex as a secondary sensor for PPi in purely aqueous solution

Abstract

A new fluorescence sensor L with benzimidazo[2,1-α]benz[de]isoquinoline-7-one as the fluorophore and glutamic acid moiety as the receptor was synthesized and characterized. In 100% aqueous buffer (tris 10 mM, pH = 7.4) solution, L showed complete fluorescence quenching in the presence of Fe3+ over other competitive metal ions. It worked based on the Fe3+-induced formation of a 1 : 1 L–Fe3+ complex, producing a reverse-PET mechanism effect. The quantum yield was determined to be 0.28 and 0.04 for free L and L–Fe3+ complexes, respectively. The L–Fe3+ ensemble could serve as a subsequent sensor for PPi due to the strong attraction between Fe3+ and PPi by fluorescence recovery without the interference of the biological competitors including ATP, ADP and PO43−. The LOD calculated by 3σ/s methods was found to be as low as 8 × 10−7 M for Fe3+ and 1.53 × 10−6 M for PPi. The DFT and a solvent-dependent (H2O) TDDFT calculations also confirmed the proposed reverse-PET mechanism indirectly.

Graphical abstract: A new fluorescent sensor containing glutamic acid for Fe3+ and its resulting complex as a secondary sensor for PPi in purely aqueous solution

Supplementary files

Article information

Article type
Paper
Submitted
20 Mar 2017
Accepted
19 Apr 2017
First published
19 Apr 2017

New J. Chem., 2017,41, 4234-4240

A new fluorescent sensor containing glutamic acid for Fe3+ and its resulting complex as a secondary sensor for PPi in purely aqueous solution

P. Li and Y. Wang, New J. Chem., 2017, 41, 4234 DOI: 10.1039/C7NJ00913E

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