Issue 17, 2017

A turn-on fluorescence aptasensor based on carbon dots for sensitive detection of adenosine

Abstract

In this paper, a novel turn-on fluorescence aptasensor has been designed for adenosine detection based on fluorescence resonance energy transfer (FRET) from single-stranded DNA labeled carbon dots (ssDNA-CDs) to aptamer modified gold nanoparticles (aptamer-AuNPs). In the absence of adenosine, the fluorescence of ssDNA-CDs was quenched by aptamer-AuNPs via the formation of an aptamer–ssDNA duplex. The introduced adenosine competed to displace ssDNA-CDs by specifically binding to the aptamer, resulting in the recovery of the quenched fluorescence of ssDNA-CDs. Under optimized conditions, the increase in fluorescence intensity was proportional to the concentration of adenosine with a linear range of 10–500 nM and allowed a limit of detection as low as 4.2 nM. In addition, this method was successfully applied to adenosine determination in human serum samples with satisfactory results.

Graphical abstract: A turn-on fluorescence aptasensor based on carbon dots for sensitive detection of adenosine

Supplementary files

Article information

Article type
Paper
Submitted
03 Jul 2017
Accepted
18 Jul 2017
First published
19 Jul 2017

New J. Chem., 2017,41, 9230-9235

A turn-on fluorescence aptasensor based on carbon dots for sensitive detection of adenosine

X. Shen, L. Xu, W. Zhu, B. Li, J. Hong and X. Zhou, New J. Chem., 2017, 41, 9230 DOI: 10.1039/C7NJ02384G

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