Issue 18, 2017

Experimental separation steps influence the protein content of corona around mesoporous silica nanoparticles

Abstract

In order to direct nanocarriers to their targets efficiently, we have to understand the interactions occurring at the nano-bio interface between nanocarriers and human proteins, which forms the layer called the corona. However, experiments aiming to identify and quantify the proteins in the corona, especially critical steps in the separation of nanoparticles from biological media may affect the corona composition. Here, we used nano-LC MS/MS to compare the protein corona contents obtained after using two different separation methods. We showed that applying centrifugation versus magnetization to isolate nanoparticles surrounded by a corona resulted in protein loss and a reshuffling of their respective abundances.

Graphical abstract: Experimental separation steps influence the protein content of corona around mesoporous silica nanoparticles

Supplementary files

Article information

Article type
Communication
Submitted
07 Mar 2017
Accepted
12 Apr 2017
First published
13 Apr 2017

Nanoscale, 2017,9, 5769-5772

Experimental separation steps influence the protein content of corona around mesoporous silica nanoparticles

C. Pisani, J. C. Gaillard, C. Dorandeu, C. Charnay, Y. Guari, J. Chopineau, J. M. Devoisselle, J. Armengaud and O. Prat, Nanoscale, 2017, 9, 5769 DOI: 10.1039/C7NR01654A

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