Issue 36, 2017

Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

Abstract

In this contribution, we designed four types of immuno-magnetic nanoparticles for separation of different T cells (CD3+, CD4+, CD8+ and CD14+ T cells), and we established a new large-scale immuno-magnetic cell sorting procedure to achieve an enrichment of particular T cells using our designed auto-IMACS device. This device could achieve recyclable large-scale cell sorting, for which the throughput of the system reached ∼4000 mL and the maximum cell capacity was 4 × 1010. The collected cells were analyzed by flow cytometry and visual cytology data, and the effective selection rates of CD3+, CD4+, CD8+ and CD14+ T cells were 79.3%, 74.1%, 57.1% and 67.9%, respectively. The sorted CD8+ T cells still retained good cytotoxic activity against specific cells. In addition, the sorted T cells can also be further incubated in vitro and proliferated, and even could be infused back into patients for immunotherapy in the near future.

Graphical abstract: Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

Article information

Article type
Paper
Submitted
07 Jul 2017
Accepted
22 Aug 2017
First published
23 Aug 2017
This article is Open Access
Creative Commons BY license

Nanoscale, 2017,9, 13592-13599

Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

Q. Zhang, T. Yin, R. Xu, W. Gao, H. Zhao, J. G. Shapter, K. Wang, Y. Shen, P. Huang, G. Gao, Y. Wu and D. Cui, Nanoscale, 2017, 9, 13592 DOI: 10.1039/C7NR04914E

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