Dioxygen insensitive C70/AuNPs hybrid system for rapid and quantitative glucose biosensing

Abstract

A novel hybrid system based on NAD-dependent glucose dehydrogenase immobilized on gold nanoparticles (AuNPs) covered with C₇₀ fullerene has been developed for effective biosensing and quantitative detection of glucose. According to amperometric measurements, the new biosensor exhibited high sensitivity (26.1 μA mM⁻¹ cm⁻²), wide linear range (0.25–12.0 mM), and rapid characteristic response (3 s). These unique properties suggest that the prepared hybrid nanocomposite is very promising to find potential application as a dioxygen insensitive biosensor for rapid and quantitative detection of glucose.