Issue 9, 2017

Using highly emissive and environmentally sensitive o-carborane-functionalized metallophosphors to monitor mitochondrial polarity

Abstract

Mitochondria as vital intracellular organelles play critical roles in multiple physiological processes, and their polarity is a crucial characteristic that can reveal the intracellular environment and impact cellular events. In this work, we designed and synthesized a novel series of highly emissive and environmentally sensitive phosphorescent iridium(III) complexes (2a–2e, 3a–3e and 4) functionalized by o-carborane. These complexes showed high emission quantum yields both in solution and in solid state (up to ΦPL = 0.82), long emission lifetime and tunable emission wavelength over 74 nm by introduction of a carboranyl motif in their ligands. Importantly, all the complexes have shown significant solvatochromic effects in contrast to the carborane-free control complex. Among them, complex 2d shows the highest sensitivity to polarity of solvents with a MPPS (maximum peak phosphorescence shift) value of 42 nm and clear dependence of phosphorescence lifetime on solvent polarity. Interestingly, complex 2d can easily penetrate into cells and preferentially distribute in mitochondria. To utilize these properties, the first phosphorescent imaging of mitochondrial polarity has been realized by photoluminescence lifetime imaging microscopy (PLIM), which can monitor mitochondria-relevant cellular processes such as cell apoptosis and distinguish cancer cells from normal cells. Compared to intensity-based sensing, lifetime-based detection is independent of the probe concentration, excitation power and photobleaching of probes, which can show high accuracy and reproducibility.

Graphical abstract: Using highly emissive and environmentally sensitive o-carborane-functionalized metallophosphors to monitor mitochondrial polarity

Supplementary files

Article information

Article type
Edge Article
Submitted
12 Jan 2017
Accepted
28 May 2017
First published
30 May 2017
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2017,8, 5930-5940

Using highly emissive and environmentally sensitive o-carborane-functionalized metallophosphors to monitor mitochondrial polarity

X. Li, X. Tong, Y. Yin, H. Yan, C. Lu, W. Huang and Q. Zhao, Chem. Sci., 2017, 8, 5930 DOI: 10.1039/C7SC00160F

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