Issue 5, 2019

Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich hybridization capture

Abstract

Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification. The assay was developed for the direct detection of RNA from dangerous human pathogens and enabled absolute and high-precision quantification of a target with a detection limit of 10 fM.

Graphical abstract: Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich hybridization capture

Supplementary files

Article information

Article type
Paper
Submitted
26 Oct 2018
Accepted
28 Dec 2018
First published
03 Jan 2019

Analyst, 2019,144, 1725-1730

Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich hybridization capture

X. Xu, J. Chen, B. Li, L. Tang and J. Jiang, Analyst, 2019, 144, 1725 DOI: 10.1039/C8AN02063A

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