Issue 89, 2018

Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

Abstract

The sparse but functionally essential post-translational collagen modification 5-hydroxylysine can undergo further transformations, including crosslinking, O-glycosylation, and glycation. Dynamic nuclear polarization (DNP) and stable isotope enriched lysine incorporation provide sufficient solid-state NMR sensitivity to identify these adducts directly in skin and vascular smooth muscle cell extracellular matrix (ECM), without extraction procedures, by comparison with chemical shifts of model compounds. Thus, DNP provides access to the elucidation of structural consequences of collagen modifications in intact tissue.

Graphical abstract: Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

Supplementary files

Article information

Article type
Communication
Submitted
21 Jun 2018
Accepted
02 Oct 2018
First published
05 Oct 2018
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 12570-12573

Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR

W. Y. Chow, R. Li, I. Goldberga, D. G. Reid, R. Rajan, J. Clark, H. Oschkinat, M. J. Duer, R. Hayward and C. M. Shanahan, Chem. Commun., 2018, 54, 12570 DOI: 10.1039/C8CC04960B

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