Biotin-tagged fluorescent sensor to visualize ‘mobile’ Zn2+ in cancer cells

Le Fang; Giuseppe Trigiante; Christina J. Kousseff; Rachel Crespo-Otero; Michael P. Philpott; Michael Watkinson

doi:10.1039/C8CC05425H

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Biotin-tagged fluorescent sensor to visualize ‘mobile’ Zn²⁺ in cancer cells†

Le Fang,

^a Giuseppe Trigiante,^b Christina J. Kousseff,

^a Rachel Crespo-Otero,

^a Michael P. Philpott^b and Michael Watkinson

*^ac

Author affiliations

* Corresponding authors

^a The Joseph Priestley Building, School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, UK

^b Centre for Cutaneous Research, Institute of Cell and Molecular Science, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London E1 2AT, UK

^c The Lennard-Jones Laboratories, School of Chemical and Physical Science, Keele University, UK
E-mail: m.watkinson@keele.ac.uk

Abstract

A cancer cell-targeting fluorescent sensor has been developed to image mobile Zn²⁺ by introducing a biotin group. It shows a highly selective response to Zn²⁺in vitro, no toxicity in cellulo and images ‘mobile’ Zn²⁺ specifically in cancer cells. We believe this probe has the potential to help improve our understanding of the role of Zn²⁺ in the processes of cancer initiation and development.

Download options Please wait...

Supplementary files

Supplementary information PDF (1862K)

Article information

DOI: https://doi.org/10.1039/C8CC05425H
Article type: Communication
Submitted: 05 Jul 2018
Accepted: 27 Jul 2018
First published: 07 Aug 2018

Download Citation

Chem. Commun., 2018,54, 9619-9622

Permissions

Request permissions

Biotin-tagged fluorescent sensor to visualize ‘mobile’ Zn²⁺ in cancer cells

L. Fang, G. Trigiante, C. J. Kousseff, R. Crespo-Otero, M. P. Philpott and M. Watkinson, Chem. Commun., 2018, 54, 9619 DOI: 10.1039/C8CC05425H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications