Issue 14, 2018

Engineering enzyme microenvironments for enhanced biocatalysis

Abstract

Protein engineering provides a means to alter protein structure leading to new functions. Much work has focused on the engineering of enzyme active sites to enhance catalytic activity, however there is an increasing trend towards engineering other aspects of biocatalysts as these efforts can also lead to useful improvements. This tutorial discusses recent advances in engineering an enzyme's local chemical and physical environment, with the goal of enhancing enzyme reaction kinetics, substrate selectivity, and activity in harsh conditions (e.g., low or high pH). By introducing stimuli-responsiveness to these enzyme modifications, dynamic control of activity also becomes possible. These new biomolecular and protein engineering techniques are separate and independent from traditional active site engineering and can therefore be applied synergistically to create new biocatalyst technologies with novel functions.

Graphical abstract: Engineering enzyme microenvironments for enhanced biocatalysis

Article information

Article type
Tutorial Review
Submitted
29 Jan 2018
First published
25 May 2018

Chem. Soc. Rev., 2018,47, 5177-5186

Author version available

Engineering enzyme microenvironments for enhanced biocatalysis

L. Lancaster, W. Abdallah, S. Banta and I. Wheeldon, Chem. Soc. Rev., 2018, 47, 5177 DOI: 10.1039/C8CS00085A

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