Issue 19, 2018

Surface topography and hydrophilicity regulate macrophage phenotype in milled microfluidic systems

Abstract

Micromilling is an underutilized technique for fabricating microfluidic platforms that is well-suited for the diverse needs of the biologic community. This technique, however, produces culture surfaces that are considerably rougher than in commercially available culture platforms and the hydrophilicity of these surfaces can vary considerably depending on the choice of material. In this study, we evaluated the impact of surface topography and hydrophilicity in milled microfluidic devices on the cellular phenotype and function of primary human macrophages. We found that the rough culture surface within micromilled systems affected the phenotype of macrophages cultured in these devices. However, the presence, type, and magnitude of this effect was dependent on the surface hydrophilicity as well as exposure to chemical polarization signals. These findings confirm that while milled microfluidic systems are an effective platform for culture and analysis of primary macrophages, the topography and hydrophilicity of the culture surface within these systems should be considered in the planning and analysis of any macrophage experiments in which phenotype is relevant.

Graphical abstract: Surface topography and hydrophilicity regulate macrophage phenotype in milled microfluidic systems

Article information

Article type
Paper
Submitted
25 Apr 2018
Accepted
27 Jul 2018
First published
17 Aug 2018

Lab Chip, 2018,18, 3011-3017

Surface topography and hydrophilicity regulate macrophage phenotype in milled microfluidic systems

D. Kosoff, J. Yu, V. Suresh, D. J. Beebe and J. M. Lang, Lab Chip, 2018, 18, 3011 DOI: 10.1039/C8LC00431E

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