Issue 43, 2018

Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

Abstract

Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.

Graphical abstract: Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

Supplementary files

Article information

Article type
Paper
Submitted
25 Jul 2018
Accepted
22 Aug 2018
First published
23 Aug 2018
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2018,16, 8056-8063

Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

A. Megia-Fernandez, B. Mills, C. Michels, S. V. Chankeshwara, N. Krstajić, C. Haslett, K. Dhaliwal and M. Bradley, Org. Biomol. Chem., 2018, 16, 8056 DOI: 10.1039/C8OB01790E

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