Issue 8, 2019

Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

Abstract

After entering a cell, most small molecule fluorescent probes are dispersed in the cytoplasm before they then accumulate in a specific organelle or subcellular zone. Molecules that can enter two or more organelles with high selectivity are all but unknown. In this work, we report a naphthalimide-based fluorescent probe, NIM-7, that allows lipid droplets and lysosomes to be labelled simultaneously and with high specificity. These subcellular entities can then be visualized readily through yellow and red fluorescence, using different excitation and detection channels. NIM-7 allows 3D imaging and quantitative visualizing of lipid droplets and lysosomes. It is also able to track simultaneously the movement of lipid droplets and lysosomes in real-time. We also report here that NIM-7 can be used to image both different cell lines and zebrafish embryos.

Graphical abstract: Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

Supplementary files

Article information

Article type
Edge Article
Submitted
08 Oct 2018
Accepted
21 Dec 2018
First published
27 Dec 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2019,10, 2342-2348

Simultaneous dual-colour tracking lipid droplets and lysosomes dynamics using a fluorescent probe

X. Zheng, W. Zhu, F. Ni, H. Ai, S. Gong, X. Zhou, Jonathan L. Sessler and C. Yang, Chem. Sci., 2019, 10, 2342 DOI: 10.1039/C8SC04462G

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements