Issue 24, 2018

A rhodamine-based fast and selective fluorescent probe for monitoring exogenous and endogenous nitric oxide in live cells

Abstract

Nitric oxide, an important cellular messenger molecule, plays a wide range of physiological roles in neurotransmission, vascular relaxation, platelet activation, and immune response. In this work, we synthesized a selective and sensitive cationic fluorescent probe (ROPD) for monitoring exogenous and endogenous nitric oxide in live cells by grafting a NO-trapper o-phenylenediamine (OPD), via one of the amino groups of OPD, onto a rhodamine fluorophore at the C-3 position. The probe exhibited high selectivity and sensitivity towards NO with fast response in the water phase over a wide pH range. At the same time, the probe ROPD is cell-membrane permeable and can be used for the real-time visualization of exogenous NO in both L929 and HeLa cells, and endogenous NO in stressed RAW 264.7 cells.

Graphical abstract: A rhodamine-based fast and selective fluorescent probe for monitoring exogenous and endogenous nitric oxide in live cells

Supplementary files

Article information

Article type
Paper
Submitted
09 Mar 2018
Accepted
18 May 2018
First published
18 May 2018

J. Mater. Chem. B, 2018,6, 4096-4103

A rhodamine-based fast and selective fluorescent probe for monitoring exogenous and endogenous nitric oxide in live cells

Q. Wang, X. Jiao, C. Liu, S. He, L. Zhao and X. Zeng, J. Mater. Chem. B, 2018, 6, 4096 DOI: 10.1039/C8TB00646F

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