Issue 62, 2019
From the journal:

Chemical Communications

4′-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading†

Ganesh N. Nawale, ORCID logo a   Saeed Bahadorikhalili,a   Pallabi Sengupta,b   Sandeep Kadekar,a   Subhrangsu Chatterjeeb  and  Oommen P. Varghese ORCID logo *a  

* Corresponding authors

a Translational Chemical Biology Laboratory, Division of Polymer Chemistry, Department of Chemistry-Ångström, Uppsala University, Uppsala, Sweden
E-mail: oommen.varghese@kemi.uu.se

b Biomolecular NMR and Drug Design Laboratory, Department of Biophysics, Bose Institute, P-1/12 CIT Scheme VII M, Kolkata, India

Abstract

We designed novel 4′-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in ≈4.9-fold more efficient knockdown than unmodified siRNA.

Graphical abstract: 4′-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading

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Article information

DOI
https://doi.org/10.1039/C9CC04141A
Article type
Communication
Submitted
29 May 2019
Accepted
05 Jul 2019
First published
05 Jul 2019
This article is Open Access
Creative Commons BY license

Chem. Commun., 2019,55, 9112-9115

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4′-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense strand loading

G. N. Nawale, S. Bahadorikhalili, P. Sengupta, S. Kadekar, S. Chatterjee and O. P. Varghese, Chem. Commun., 2019, 55, 9112 DOI: 10.1039/C9CC04141A

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