Issue 61, 2019

Ligase amplification reaction-catalyzed assembly of a single quantum dot-based nanosensor for sensitive detection of alkaline phosphatase

Abstract

We develop a simple method for sensitive detection of alkaline phosphatase (ALP) based on the ligase amplification reaction-catalyzed assembly of a single quantum dot (QD)-based nanosensor. This nanosensor requires only a single ligase enzyme to achieve ultrahigh sensitivity with a detection limit of 5.63 × 10−7 U mL−1, and can be applied for kinetic analysis, inhibitor screening, and ALP measurement in cell extracts.

Graphical abstract: Ligase amplification reaction-catalyzed assembly of a single quantum dot-based nanosensor for sensitive detection of alkaline phosphatase

Supplementary files

Article information

Article type
Communication
Submitted
07 Jun 2019
Accepted
02 Jul 2019
First published
02 Jul 2019

Chem. Commun., 2019,55, 8963-8966

Ligase amplification reaction-catalyzed assembly of a single quantum dot-based nanosensor for sensitive detection of alkaline phosphatase

F. Ma, M. Liu and C. Zhang, Chem. Commun., 2019, 55, 8963 DOI: 10.1039/C9CC04369A

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