Issue 44, 2019

Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes

Abstract

There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we report a simple and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines.

Graphical abstract: Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes

Supplementary files

Article information

Article type
Paper
Submitted
27 Feb 2019
Accepted
15 Oct 2019
First published
29 Oct 2019
This article is Open Access
Creative Commons BY-NC license

Nanoscale, 2019,11, 21317-21323

Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes

P. K. Jain, J. H. Lo, S. Rananaware, M. Downing, A. Panda, M. Tai, S. Raghavan, H. E. Fleming and S. N. Bhatia, Nanoscale, 2019, 11, 21317 DOI: 10.1039/C9NR01786K

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