A novel electrochemical biosensor for ultrasensitive Hg2+ detection via a triple signal amplification strategy

Wang He; Bin Qiao; Fengzhen Li; Lisha Pan; Delun Chen; Yang Cao; Jinchun Tu; Xiaohong Wang; Chuanzhu Lv; Qiang Wu

doi:10.1039/D0CC07268K

A novel electrochemical biosensor for ultrasensitive Hg²⁺ detection via a triple signal amplification strategy†

Wang He,^a Bin Qiao,^b Fengzhen Li,^a Lisha Pan,^a Delun Chen,^ab Yang Cao,^ac Jinchun Tu,

^a Xiaohong Wang,

*^a Chuanzhu Lv*^d and Qiang Wu

*^b

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* Corresponding authors

^a State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, China
E-mail: wangxiaohong@hainu.edu.cn
Fax: +86 898 66259764
Tel: +86 898 66259764

^b School of Tropical Medicine and Laboratory Medicine, Key Laboratory of Emergency and Trauma of Ministry of Education, Research Unit of Island Emergency Medicine, Chinese Academy of Medical Sciences (No. 2019RU013), Hainan Medical University, Haikou, China
E-mail: wuqiang001001@aliyun.com
Fax: 898 66984599
Tel: +86 898 66984599

^c Qiongtai Normal University, Haikou, China

^d Department of Emergency, Hainan Clinical Research Center for Acute and Critical Diseases, The Second Affiliated Hospital of Hainan Medical University, Emergency and Trauma College, Hainan Medical University, Haikou, China
E-mail: lvchuanzhu677@126.com
Fax: +86 898 66989196
Tel: +86 898 66989196

Abstract

We developed a novel electrochemical biosensor for ultrasensitive Hg²⁺ detection via a triple signal amplification strategy of a DNA dual cycle, organic–inorganic hybrid nanoflowers (Cu₃(PO₄)₂ HNFs) and gold nanoparticle (AuNP) probe. The DNA dual cycle was triggered by exonuclease III (Exo III) in the presence of Hg²⁺, and Cu₃(PO₄)₂ HNFs were synthesized as an AuNP probe carrier. The electrochemical biosensor displayed high stability, high sensitivity and excellent specificity, which was improved by up to seven orders of magnitude compared to the World Health Organization (WHO) allowed Hg²⁺ levels in drinking water. This signal amplification strategy could be easily modified and extended to detect other hazardous heavy metals and nucleic acids.

Chemical Communications

A novel electrochemical biosensor for ultrasensitive Hg²⁺ detection via a triple signal amplification strategy†

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A novel electrochemical biosensor for ultrasensitive Hg²⁺ detection via a triple signal amplification strategy

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Chemical Communications