Engineered protein cages for selective heparin encapsulation

Salla Välimäki; Qing Liu; Lise Schoonen; Daan F. M. Vervoort; Nonappa; Veikko Linko; Roeland J. M. Nolte; Jan C. M. van Hest; Mauri A. Kostiainen

doi:10.1039/D0TB02541K

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Engineered protein cages for selective heparin encapsulation†

Salla Välimäki,‡^a Qing Liu,‡^a Lise Schoonen,

^b Daan F. M. Vervoort,^c Nonappa,

^d Veikko Linko,

^ad Roeland J. M. Nolte,

^b Jan C. M. van Hest

^bc and Mauri A. Kostiainen

*^ad

Author affiliations

* Corresponding authors

^a Department of Bioproducts and Biosystems, Aalto University, P.O. Box 16100, Aalto FI-00076, Espoo, Finland
E-mail: mauri.kostiainen@aalto.fi

^b Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, Nijmegen 6525 AJ, The Netherlands

^c Department of Bio-Organic Chemistry, Eindhoven University of Technology, Institute of Complex Molecular Systems (ICMS), Het Kranenveld 14, Eindhoven 5600 MB, The Netherlands

^d HYBER Centre, Department of Applied Physics, Aalto University, Aalto FI-00076, Finland

Abstract

A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. The encapsulation is specific and the capsid-heparin assemblies display negligible hemolytic activity, indicating proper blood compatibility and promising possibilities for heparin antidote applications.

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Article information

DOI: https://doi.org/10.1039/D0TB02541K
Article type: Communication
Submitted: 27 Oct 2020
Accepted: 16 Dec 2020
First published: 17 Dec 2020
This article is Open Access

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J. Mater. Chem. B, 2021,9, 1272-1276

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Engineered protein cages for selective heparin encapsulation

S. Välimäki, Q. Liu, L. Schoonen, D. F. M. Vervoort, Nonappa, V. Linko, R. J. M. Nolte, J. C. M. van Hest and M. A. Kostiainen, J. Mater. Chem. B, 2021, 9, 1272 DOI: 10.1039/D0TB02541K

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Journal of Materials Chemistry B

Engineered protein cages for selective heparin encapsulation†

Abstract

Supplementary files

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Engineered protein cages for selective heparin encapsulation

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