Sensitive, general and portable detection of RNAs combining duplex-specific nuclease transduction with an off-shelf signalling platform

Abstract

We report a novel RNA sensing platform by combining the RNA:DNA hybridization, duplex-specific nuclease (DSN) amplification, and personal glucose meter (PGM) readout. The so-called DSN–PGM sensing platform is sensitive, specific and general to both microRNA and long virus RNA. The detection procedure is simple and instrument-free, thus holding particular potential in the development of portable and point-of-care measurements.