Chemistry of micrococcin P. Part XI. Application of a simple micromethod for the identification of lower volatile fatty acids to the structural study of 2-acylthiazoles

Abstract

An acyl group attached to the 2-position of the thiazole ring is removed hydrolytically by hot mineral acid generating the related carboxylic acid which is identified as its p-nitrobenzyl ester by t.l.c. Direct chemical evidence has thus been obtained for the presence of a propionyl group in dimethyl micrococcinate. The procedure is applicable down to about the 0·5 µmole scale.