Biosynthetic studies on pseudomonic acid (mupirocin), a novel antibiotic metabolite of Pseudomonas fluorescens
Abstract
2 H and 18O Isotope shifts observed in the 13C n.m.r. spectra of pseudomonic acid (mupirocin) enriched from [1-13C, 2H3]- and [1-13C, 18O2]-acetates provide information on the mechanisms of formation of the tetrahydropyran and ester functions. The results of incorporation studies with 14C- and 13C2– labelled β-hydroxy-β-methylglutarates do not support previous proposals for its involvement in the biosynthesis of pseudomonic acid.