Issue 47, 2007

Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

Abstract

5′-Amino-5′-deoxyguanosine-5′-N-phosphoramidate (GNHP) was synthesized in four steps from guanosine and was found to initiate T7 RNAP-promoted transcriptions to afford 5′-H2N-RNA that can be conjugated to activated esters.

Graphical abstract: Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

Article information

Article type
Communication
Submitted
06 Aug 2007
Accepted
19 Sep 2007
First published
11 Oct 2007

Chem. Commun., 2007, 5096-5098

Synthesis of 5′-amino-5′-deoxyguanosine-5′-N-phosphoramidate and its enzymatic incorporation at the 5′-termini of RNA molecules

D. Williamson, M. J. Cann and D. R. W. Hodgson, Chem. Commun., 2007, 5096 DOI: 10.1039/B712066D

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