Issue 5, 2008

Synthesis of guanosine 5′-conjugates and their use as initiator molecules for transcription priming

Abstract

We have synthesised two guanosine derivatives that are linked to biotinylated adenosine moieties by using two different strategies, one that includes synthetic steps on the solid phase and another one that is performed entirely in solution. The synthesised derivatives were shown to function as initiator molecules in transcription priming experiments. The incorporation efficiency was determined to be approximately 2%. Even though this value is rather low, the use of either molecule in selection experiments seems reasonable. Basically, RNA libraries with sequence complexities of 1015 to 1016 can be generated. Labelling of such a library with our initiator molecule would still produce 1013 to 1014 labelled/functionalised sequences, and thus sufficient sequence space for selection.

Graphical abstract: Synthesis of guanosine 5′-conjugates and their use as initiator molecules for transcription priming

Supplementary files

Article information

Article type
Paper
Submitted
19 Oct 2007
Accepted
19 Dec 2007
First published
23 Jan 2008

Org. Biomol. Chem., 2008,6, 899-907

Synthesis of guanosine 5′-conjugates and their use as initiator molecules for transcription priming

J. Wolf, V. Dombos, B. Appel and S. Müller, Org. Biomol. Chem., 2008, 6, 899 DOI: 10.1039/B716151D

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