Issue 21, 2008
From the journal:

Chemical Communications

Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates†

Filip Kielar,a   Aileen Congreve,a   Ga-lai Law,a   Elizabeth J. New,a   David Parker,*a   Ka-Leung Wong,b   Pilar Castreňoc  and  Javier de Mendozac  

* Corresponding authors

a Department of Chemistry, Durham University, South Road, Durham, UK
E-mail: david.parker@dur.ac.uk

b Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong

c Institute of Chemical Research of Catalonia (ICIQ), Avgda. Països Catalans 16, Tarragona, Spain

Abstract

An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolicintracellular distributions.

Graphical abstract: Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

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Article information

DOI
https://doi.org/10.1039/B803864C
Article type
Communication
Submitted
05 Mar 2008
Accepted
26 Mar 2008
First published
22 Apr 2008

Chem. Commun., 2008, 2435-2437

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Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

F. Kielar, A. Congreve, G. Law, E. J. New, D. Parker, K. Wong, P. Castreňo and J. de Mendoza, Chem. Commun., 2008, 2435 DOI: 10.1039/B803864C

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