Issue 14, 2009

A three-phase microfluidic chip for rapid sample clean-up of alkaloids from plant extracts

Abstract

A three-phase microchip was developed for the rapid and efficient small-scale purification of alkaloids from plant extracts. As part of the development of such a three-phase microchip, first a two-phase microchip with two channels (3.2 cm and 9.3 cm) was used to study the extraction efficiency of strychnine nitrate and strychnine at various flow rates. Strychnine was extracted from a basic aqueous phase to a chloroform phase (extraction) or strychnine was extracted from a chloroform phase into an acidic aqueous phase (back extraction). Subsequently, the “simultaneous extraction and back extraction” of strychnine was carried out in a three-phase microchip. The experimental extraction rate and yield were compared with model data. At a residence time of 25 sec, 79.5% of strychnine was extracted into the acidic aqueous phase using the three-phase microchip. In general, a good correlation was found between experimental results and model data for both two- and three-phase extractions. Finally, the three-phase microchip was employed in the purification of alkaloids (strychnine and brucine) from Strychnos seed extracts.

Graphical abstract: A three-phase microfluidic chip for rapid sample clean-up of alkaloids from plant extracts

Supplementary files

Article information

Article type
Paper
Submitted
09 Dec 2008
Accepted
25 Mar 2009
First published
14 Apr 2009

Lab Chip, 2009,9, 2085-2092

A three-phase microfluidic chip for rapid sample clean-up of alkaloids from plant extracts

K. K. R. Tetala, J. W. Swarts, B. Chen, A. E. M. Janssen and T. A. van Beek, Lab Chip, 2009, 9, 2085 DOI: 10.1039/B822106E

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