Issue 8, 2010
From the journal:

Molecular BioSystems

Immobilization of trypsin on water-soluble dendrimer-modified carbon nanotubes for on-plate proteolysis combined with MALDI-MS analysis†

Ying Zhang,‡a   Weiman Cao,‡b   Minbo Liu,a   Shiping Yang,b   Huixia Wu,*b   Haojie Lu*a  and  Pengyuan Yanga  

* Corresponding authors

a Institutes of Biomedical Sciences and Department of Chemistry, Fudan University, Shanghai 200433, P. R. China
E-mail: luhaojie@fudan.edu.cn
Fax: (+86) 21-5423-7618

b Department of Chemistry, Shanghai Normal University, Shanghai 200234, P. R. China
E-mail: wuhuixia@shnu.edu.cn

Abstract

A novel on-plate digestion method combined with MALDI-MS analysis is reported, using trypsin-linked dendrimer-modified carbon nanotubes (dCNTs) as the enzyme immobilization probe. Excellent digestion performance was achieved in a short time without any complicated reduction and alkylation procedures.

Graphical abstract: Immobilization of trypsin on water-soluble dendrimer-modified carbon nanotubes for on-plate proteolysis combined with MALDI-MS analysis

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Article information

DOI
https://doi.org/10.1039/C004911E
Article type
Communication
Submitted
07 Apr 2010
Accepted
19 May 2010
First published
09 Jun 2010

Mol. BioSyst., 2010,6, 1447-1449

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Immobilization of trypsin on water-soluble dendrimer-modified carbon nanotubes for on-plate proteolysis combined with MALDI-MS analysis

Y. Zhang, W. Cao, M. Liu, S. Yang, H. Wu, H. Lu and P. Yang, Mol. BioSyst., 2010, 6, 1447 DOI: 10.1039/C004911E

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