Issue 4, 2018

Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

Abstract

The development of a rapid and chemoselective selenocystine–selenoester peptide ligation that operates at nanomolar reactant concentrations has been developed by utilising PNA templation. Kinetic analysis of the templated peptide ligation revealed that the selenocystine–selenoester reaction was 10 times faster than traditional native chemical ligation at cysteine and to our knowledge is the fastest templated ligation reaction reported to date. The efficiency and operational simplicity of this technology is highlighted through the formation of hairpin molecular architectures and in a novel paper-based lateral flow assay for the rapid and sequence specific detection of oligonucleotides, including miRNA in cell lysates.

Graphical abstract: Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

Supplementary files

Article information

Article type
Edge Article
Submitted
20 Jun 2017
Accepted
21 Nov 2017
First published
22 Nov 2017
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2018,9, 896-903

Peptide nucleic acid-templated selenocystine–selenoester ligation enables rapid miRNA detection

J. Sayers, R. J. Payne and N. Winssinger, Chem. Sci., 2018, 9, 896 DOI: 10.1039/C7SC02736B

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