2JM6

Solution structure of MCL-1 complexed with NOXAB


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 256 
  • Conformers Submitted: 20 
  • Selection Criteria: lowest energy, least restraint violations, structures with acceptable covalent geometry 

wwPDB Validation   3D Report Full Report


This is version 1.4 of the entry. See complete history


Literature

Structural insights into the degradation of Mcl-1 induced by BH3 domains

Czabotar, P.E.Lee, E.F.van Delft, M.F.Day, C.L.Smith, B.J.Huang, D.C.S.Fairlie, W.D.Hinds, M.G.Colman, P.M.

(2007) Proc Natl Acad Sci U S A 104: 6217-6222

  • DOI: https://doi.org/10.1073/pnas.0701297104
  • Primary Citation of Related Structures:  
    2JM6, 2NL9, 2NLA

  • PubMed Abstract: 

    Apoptosis is held in check by prosurvival proteins of the Bcl-2 family. The distantly related BH3-only proteins bind to and antagonize them, thereby promoting apoptosis. Whereas binding of the BH3-only protein Noxa to prosurvival Mcl-1 induces Mcl-1 degradation by the proteasome, binding of another BH3-only ligand, Bim, elevates Mcl-1 protein levels. We compared the three-dimensional structures of the complexes formed between BH3 peptides of both Bim and Noxa, and we show that a discrete C-terminal sequence of the Noxa BH3 is necessary to instigate Mcl-1 degradation.


  • Organizational Affiliation

    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Noxa27Mus musculusMutation(s): 0 
Gene Names: Pmaip1Noxa
UniProt
Find proteins for Q9JM54 (Mus musculus)
Explore Q9JM54 
Go to UniProtKB:  Q9JM54
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupQ9JM54
Sequence Annotations
Expand
  • Reference Sequence
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
Myeloid cell leukemia-1 protein Mcl-1 homolog162Mus musculusMutation(s): 0 
Gene Names: Mcl1
UniProt & NIH Common Fund Data Resources
Find proteins for P97287 (Mus musculus)
Explore P97287 
Go to UniProtKB:  P97287
IMPC:  MGI:101769
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP97287
Sequence Annotations
Expand
  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 256 
  • Conformers Submitted: 20 
  • Selection Criteria: lowest energy, least restraint violations, structures with acceptable covalent geometry 

Structure Validation

View Full Validation Report



Entry History 

Deposition Data

Revision History  (Full details and data files)

  • Version 1.0: 2007-03-20
    Type: Initial release
  • Version 1.1: 2007-10-21
    Changes: Version format compliance
  • Version 1.2: 2011-07-13
    Changes: Version format compliance
  • Version 1.3: 2022-03-09
    Changes: Data collection, Database references, Derived calculations
  • Version 1.4: 2023-12-20
    Changes: Data collection, Other