2WF8
Structure of Beta-Phosphoglucomutase inhibited with Glucose-6- phosphate, Glucose-1-phosphate and Beryllium trifluoride
- PDB DOI: https://doi.org/10.2210/pdb2WF8/pdb
- Classification: ISOMERASE
- Organism(s): Lactococcus lactis
- Expression System: Escherichia coli BL21
- Mutation(s): No
- Deposited: 2009-04-03 Released: 2010-05-26
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.20 Å
- R-Value Free: 0.191
- R-Value Work: 0.162
- R-Value Observed: 0.163
This is version 4.2 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| BETA-PHOSPHOGLUCOMUTASE | 221 | Lactococcus lactis | Mutation(s): 0 EC: 5.4.2.6 |  | |
UniProt | |||||
Find proteins for P71447 (Lactococcus lactis subsp. lactis (strain IL1403)) Explore P71447 Go to UniProtKB: P71447 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P71447 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 6 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| BG6 Query on BG6 | E [auth A] | 6-O-phosphono-beta-D-glucopyranose C6 H13 O9 P NBSCHQHZLSJFNQ-VFUOTHLCSA-N |  | ||
| G1P Query on G1P | D [auth A] | 1-O-phosphono-alpha-D-glucopyranose C6 H13 O9 P HXXFSFRBOHSIMQ-VFUOTHLCSA-N |  | ||
| PEG Query on PEG | H [auth A], I [auth A], J [auth A] | DI(HYDROXYETHYL)ETHER C4 H10 O3 MTHSVFCYNBDYFN-UHFFFAOYSA-N |  | ||
| BEF Query on BEF | B [auth A] | BERYLLIUM TRIFLUORIDE ION Be F3 OGIAHMCCNXDTIE-UHFFFAOYSA-K |  | ||
| MG Query on MG | C [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
| NA Query on NA | F [auth A], G [auth A] | SODIUM ION Na FKNQFGJONOIPTF-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.20 Å
- R-Value Free: 0.191
- R-Value Work: 0.162
- R-Value Observed: 0.163
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 37.3 | α = 90 |
| b = 54.3 | β = 90 |
| c = 104.2 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
| MOLREP | phasing |
Entry History
Deposition Data
- Released Date: 2010-05-26 Deposition Author(s): Bowler, M.W., Baxter, N.J., Webster, C.E., Pollard, S., Alizadeh, T., Hounslow, A.M., Cliff, M.J., Bermel, W., Williams, N.H., Hollfelder, F., Blackburn, G.M., Waltho, J.P.
Revision History (Full details and data files)
- Version 1.0: 2010-05-26
Type: Initial release - Version 1.1: 2011-09-21
Changes: Atomic model, Derived calculations, Non-polymer description, Other, Version format compliance - Version 1.2: 2012-05-16
Changes: Other - Version 2.0: 2019-01-30
Changes: Advisory, Atomic model, Data collection, Database references, Experimental preparation - Version 3.0: 2019-05-22
Changes: Advisory, Atomic model, Data collection - Version 4.0: 2019-10-02
Changes: Atomic model, Data collection, Other - Version 4.1: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Derived calculations, Structure summary - Version 4.2: 2023-12-13
Changes: Data collection, Database references, Refinement description, Structure summary
