3UON
Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist
- PDB DOI: https://doi.org/10.2210/pdb3UON/pdb
- Classification: SIGNALING PROTEIN/ANTAGONIST
- Organism(s): Homo sapiens, Tequatrovirus T4
- Expression System: Spodoptera frugiperda
- Mutation(s): Yes
- Membrane Protein: Yes OPMPDBTMMemProtMDmpstruc
- Deposited: 2011-11-16 Released: 2012-02-01
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 3.00 Å
- R-Value Free: 0.276
- R-Value Work: 0.225
- R-Value Observed: 0.227
This is version 1.4 of the entry. See complete history.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Human M2 muscarinic acetylcholine, receptor T4 lysozyme fusion protein | 467 | Homo sapiens, Tequatrovirus T4 | Mutation(s): 6 Gene Names: CHRM2, E EC: 3.2.1.17 Membrane Entity: Yes |  | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for P08172 (Homo sapiens) Explore P08172 Go to UniProtKB: P08172 | |||||
PHAROS: P08172 GTEx: ENSG00000181072 | |||||
Find proteins for P00720 (Enterobacteria phage T4) Explore P00720 Go to UniProtKB: P00720 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Groups | P00720P08172 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| QNB Query on QNB | B [auth A] | (3R)-1-azabicyclo[2.2.2]oct-3-yl hydroxy(diphenyl)acetate C21 H23 N O3 HGMITUYOCPPQLE-IBGZPJMESA-N |  | ||
| BGC Query on BGC | C [auth A] | beta-D-glucopyranose C6 H12 O6 WQZGKKKJIJFFOK-VFUOTHLCSA-N |  | ||
| CL Query on CL | D [auth A], E [auth A] | CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 3.00 Å
- R-Value Free: 0.276
- R-Value Work: 0.225
- R-Value Observed: 0.227
- Space Group: P 1 21 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 78.17 | α = 90 |
| b = 47.26 | β = 109.7 |
| c = 88.12 | γ = 90 |
| Software Name | Purpose |
|---|---|
| HKL-2000 | data collection |
| PHASER | phasing |
| PHENIX | refinement |
| HKL-2000 | data reduction |
| HKL-2000 | data scaling |
Entry History
Deposition Data
- Released Date: 2012-02-01 Deposition Author(s): Haga, K., Kruse, A.C., Asada, H., Yurugi-Kobayashi, T., Shiroishi, M., Zhang, C., Weis, W.I., Okada, T., Kobilka, B.K., Haga, T., Kobayashi, T.
Revision History (Full details and data files)
- Version 1.0: 2012-02-01
Type: Initial release - Version 1.1: 2012-03-28
Changes: Database references - Version 1.2: 2017-08-09
Changes: Refinement description, Source and taxonomy - Version 1.3: 2020-07-29
Type: Remediation
Reason: Carbohydrate remediation
Changes: Data collection, Database references, Derived calculations, Structure summary - Version 1.4: 2023-09-13
Changes: Data collection, Database references, Refinement description, Structure summary
