5X29

NMR structure of the SARS Coronavirus E protein pentameric ion channel


Experimental Data Snapshot

  • Method: SOLUTION NMR
  • Conformers Calculated: 200 
  • Conformers Submitted: 16 
  • Selection Criteria: structures with the lowest energy 

wwPDB Validation   3D Report Full Report


This is version 2.1 of the entry. See complete history


Literature

Structural model of the SARS coronavirus E channel in LMPG micelles

Surya, W.Li, Y.Torres, J.

(2018) Biochim Biophys Acta 1860: 1309-1317

  • DOI: https://doi.org/10.1016/j.bbamem.2018.02.017
  • Primary Citation of Related Structures:  
    5X29

  • PubMed Abstract: 

    Coronaviruses (CoV) cause common colds in humans, but are also responsible for the recent Severe Acute, and Middle East, respiratory syndromes (SARS and MERS, respectively). A promising approach for prevention are live attenuated vaccines (LAVs), some of which target the envelope (E) protein, which is a small membrane protein that forms ion channels. Unfortunately, detailed structural information is still limited for SARS-CoV E, and non-existent for other CoV E proteins. Herein, we report a structural model of a SARS-CoV E construct in LMPG micelles with, for the first time, unequivocal intermolecular NOEs. The model corresponding to the detergent-embedded region is consistent with previously obtained orientational restraints obtained in lipid bilayers and in vivo escape mutants. The C-terminal domain is mostly α-helical, and extramembrane intermolecular NOEs suggest interactions that may affect the TM channel conformation.


  • Organizational Affiliation

    School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.


Macromolecules
Find similar proteins by:  (by identity cutoff)  |  3D Structure
Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
Envelope small membrane protein
A, B, C, D, E
81Severe acute respiratory syndrome-related coronavirusMutation(s): 3 
Gene Names: EsM4
Membrane Entity: Yes 
UniProt
Find proteins for P59637 (Severe acute respiratory syndrome coronavirus)
Explore P59637 
Go to UniProtKB:  P59637
Entity Groups  
Sequence Clusters30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt GroupP59637
Sequence Annotations
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  • Reference Sequence
Experimental Data & Validation

Experimental Data

  • Method: SOLUTION NMR
  • Conformers Calculated: 200 
  • Conformers Submitted: 16 
  • Selection Criteria: structures with the lowest energy 

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
Ministry of EducationSingaporeRG 51/13

Revision History  (Full details and data files)

  • Version 1.0: 2017-06-07
    Type: Initial release
  • Version 2.0: 2018-07-04
    Changes: Data collection, Database references, Polymer sequence
  • Version 2.1: 2023-06-14
    Changes: Data collection, Database references, Other