Issue 7, 2005
From the journal:

Physical Chemistry Chemical Physics

Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

Jos Oomens,a   Nick Polfer,a   David T. Moore,a   Lex van der Meer,a   Alan G. Marshall,bc   John R. Eyler,d   Gerard Meijere  and  Gert von Helden*e  

* Corresponding authors

a FOM Institute for Plasma Physics Rijnhuizen, Edisonbaan 14, Nieuwegein, The Netherlands

b National High Magnetic Field Laboratory, 1800 E. Paul Dirac Drive, Tallahassee, USA

c Department of Chemistry and Biochemistry, Florida State University, Tallahassee, USA

d Department of Chemistry, University of Florida, Gainesville, USA

e Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, Berlin, Germany
E-mail: helden@fhi-berlin.mpg.de
Fax: +49-30-84135600
Tel: +49-30-84135603

Abstract

Infrared spectra of a 104 amino-acid protein in the gas phase as a function of its charge state are presented. The spectra contain clearly resolvable bands in the amide I and II spectral regions, as well as a band at 1483 cm−1, which is not observed in solution phase spectroscopy and is especially prominent for the higher charge states. Compared to solution, the amide I band is blue-shifted and the amide II band red-shifted, as expected for species in an environment with reduced hydrogen bonding. The band positions are suggestive of a mostly α-helical structure of the protein and their widths are comparable to those in solution, suggesting a similar conformational distribution.

Graphical abstract: Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

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Article information

DOI
https://doi.org/10.1039/B502322J
Article type
Communication
Submitted
15 Feb 2005
Accepted
03 Mar 2005
First published
07 Mar 2005

Phys. Chem. Chem. Phys., 2005,7, 1345-1348

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Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

J. Oomens, N. Polfer, D. T. Moore, L. van der Meer, A. G. Marshall, J. R. Eyler, G. Meijer and G. von Helden, Phys. Chem. Chem. Phys., 2005, 7, 1345 DOI: 10.1039/B502322J

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