Nanopore single-molecule investigation of aflatoxin B1–aptamer interactions for evolving the aptamer

Yongqi Yang; Chunmeng Zhong; Junjie Huang; Jianyuan Dai; Cuisong Zhou

doi:10.1039/D5CC00623F

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Nanopore single-molecule investigation of aflatoxin B1–aptamer interactions for evolving the aptamer†

Yongqi Yang,^a Chunmeng Zhong,^a Junjie Huang,^a Jianyuan Dai

^a and Cuisong Zhou

*^a

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* Corresponding authors

^a College of Chemistry, Sichuan University, Chengdu 610064, P. R. China
E-mail: zcs@scu.edu.cn

Abstract

By utilizing characteristic blocking signatures, the interactions between aflatoxin B1 (AFB1) and its aptamer were investigated. Furthermore, its aptamer sequence was evolved to have a higher affinity (K_d) calculated to be 18.74 nM for AFB1 than previous reports. This evolved aptamer-based nanopore strategy enabled the sensitive detection of trace AFB1 with a linear range from 0.5 to 100 nM.

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DOI: https://doi.org/10.1039/D5CC00623F
Article type: Communication
Submitted: 04 Feb 2025
Accepted: 14 Mar 2025
First published: 24 Mar 2025

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Chem. Commun., 2025, Advance Article

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Nanopore single-molecule investigation of aflatoxin B1–aptamer interactions for evolving the aptamer

Y. Yang, C. Zhong, J. Huang, J. Dai and C. Zhou, Chem. Commun., 2025, Advance Article , DOI: 10.1039/D5CC00623F

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Chemical Communications

Nanopore single-molecule investigation of aflatoxin B1–aptamer interactions for evolving the aptamer†

Abstract

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Nanopore single-molecule investigation of aflatoxin B1–aptamer interactions for evolving the aptamer

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